RESUMO
Iron overload contributes to the development of neurodegeneration and the exacerbation of normal apoptosis rates, largely due to its participation in the Fenton reaction and production of reactive oxygen species (ROS). Mitochondria constitute the major intracellular source of ROS and the main target of attack by free radicals. They are dynamic organelles that bind (fusion) and divide (fission) in response to environmental stimuli, developmental status, and energy needs of the cells. Sulforaphane (SFN) is a natural compound that displays antioxidant and anti-inflammatory activities. This study aims to investigate the effects of SFN on memory deficits and changes in markers of mitochondrial function, DNM1L and OPA1, and the synaptic marker, synaptophysin, induced by neonatal iron treatment. Male rats received vehicle or carbonyl iron (30mg/kg) from the 12th to the 14th postnatal day. In adulthood, they were treated with saline or SFN (0.5 or 5mg/kg) for 14days every other day. Memory deficits were assessed using the object recognition task. DNM1L, OPA1, and synaptophysin levels in the hippocampus were quantified by Western blotting. Results showed that SFN was able to reverse iron-induced decreases in mitochondrial fission protein, DNM1L, as well as synaptophysin levels in the hippocampus, leading to a recovery of recognition memory impairment induced by iron. These findings suggest that SFN may be further investigated as potential agent for the treatment of cognitive deficits associated with neurodegenerative disorders.
Assuntos
Anticarcinógenos/uso terapêutico , Compostos de Ferro/metabolismo , Isotiocianatos/uso terapêutico , Transtornos da Memória , Mitocôndrias/metabolismo , Sinapses/metabolismo , Animais , Animais Recém-Nascidos , Encéfalo/metabolismo , Encéfalo/patologia , Catalase/genética , Catalase/metabolismo , Modelos Animais de Doenças , Dinaminas/genética , Dinaminas/metabolismo , Comportamento Exploratório/efeitos dos fármacos , Feminino , GTP Fosfo-Hidrolases/genética , GTP Fosfo-Hidrolases/metabolismo , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Glutationa Peroxidase/metabolismo , Ferro/metabolismo , Compostos de Ferro/toxicidade , Masculino , Transtornos da Memória/tratamento farmacológico , Transtornos da Memória/etiologia , Transtornos da Memória/patologia , Mitocôndrias/patologia , NAD(P)H Desidrogenase (Quinona)/metabolismo , Gravidez , Ratos , Ratos Wistar , Reconhecimento Psicológico/efeitos dos fármacos , SulfóxidosRESUMO
The authors used Penrose drains around intraperitoneal portion of Silastic tubes to prevent subphrenic collections in transhepatic intubation. In 31 cases of benign stenosis of the biliary tree, this peritoneal drainage was employed in 18 and no collections were observed. In the other group, 3 patients developed subphrenic abscess. Even with a result not statistically significant (P less than 0.05), the authors consider the procedure useful in transhepatic intubation.
